reference strain c albicans atcc 90031 Search Results


c subvermispora strain  (ATCC)
93
ATCC c subvermispora strain
Schematic of pCsGi- hph and pCsbtubi- hph plasmids. Plasmids pCsGi- hph and pCsbtubi- hph were constructed by combining the homologous promoter region of gpd and beta-tubulin genes from C. <t>subvermispora</t> , respectively, with the coding sequence of bacterial hph . In pCsGi- hph , the first exon, ATGCCC, and the first intron from Csgpd were inserted just before the hygromycin phosphotransferase sequence. In pCsbtubi- hph , the first and second introns, as well as the first exon (encoding 11 amino acids), from Csß - tub were inserted just before the hygromycin phosphotransferase sequence. The plasmid backbones of pCsGi- hph and pCsbtubi- hph are pCRII (Thermo Fisher Scientific Inc.) and pUC19 (Takara Bio Inc.), respectively
C Subvermispora Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Schematic of pCsGi- hph and pCsbtubi- hph plasmids. Plasmids pCsGi- hph and pCsbtubi- hph were constructed by combining the homologous promoter region of gpd and beta-tubulin genes from C. subvermispora , respectively, with the coding sequence of bacterial hph . In pCsGi- hph , the first exon, ATGCCC, and the first intron from Csgpd were inserted just before the hygromycin phosphotransferase sequence. In pCsbtubi- hph , the first and second introns, as well as the first exon (encoding 11 amino acids), from Csß - tub were inserted just before the hygromycin phosphotransferase sequence. The plasmid backbones of pCsGi- hph and pCsbtubi- hph are pCRII (Thermo Fisher Scientific Inc.) and pUC19 (Takara Bio Inc.), respectively

Journal: AMB Express

Article Title: Stable and transient transformation, and a promoter assay in the selective lignin-degrading fungus, Ceriporiopsis subvermispora

doi: 10.1186/s13568-019-0818-1

Figure Lengend Snippet: Schematic of pCsGi- hph and pCsbtubi- hph plasmids. Plasmids pCsGi- hph and pCsbtubi- hph were constructed by combining the homologous promoter region of gpd and beta-tubulin genes from C. subvermispora , respectively, with the coding sequence of bacterial hph . In pCsGi- hph , the first exon, ATGCCC, and the first intron from Csgpd were inserted just before the hygromycin phosphotransferase sequence. In pCsbtubi- hph , the first and second introns, as well as the first exon (encoding 11 amino acids), from Csß - tub were inserted just before the hygromycin phosphotransferase sequence. The plasmid backbones of pCsGi- hph and pCsbtubi- hph are pCRII (Thermo Fisher Scientific Inc.) and pUC19 (Takara Bio Inc.), respectively

Article Snippet: The wildtype C. subvermispora strain (Fp-90031-sp (ATCC 90467) is available from ATCC, and the base plasmid used for experiments herein; pPHT1 (Cummings et al. ) was a kind gift of Professor Miriam E. Zolan, Department of Biology at Indiana University.

Techniques: Construct, Sequencing, Plasmid Preparation

Promoter analysis of C. subvermispora gpd using transformants. Promoter constructs used in deletion analysis of the Csgpd promoter, and number of drug-resistant transformants (n = 3, ± SD) grown on the first screening plate containing 200 µg/ml hygromycin B. The A nucleotide in the first ATG was counted as +1

Journal: AMB Express

Article Title: Stable and transient transformation, and a promoter assay in the selective lignin-degrading fungus, Ceriporiopsis subvermispora

doi: 10.1186/s13568-019-0818-1

Figure Lengend Snippet: Promoter analysis of C. subvermispora gpd using transformants. Promoter constructs used in deletion analysis of the Csgpd promoter, and number of drug-resistant transformants (n = 3, ± SD) grown on the first screening plate containing 200 µg/ml hygromycin B. The A nucleotide in the first ATG was counted as +1

Article Snippet: The wildtype C. subvermispora strain (Fp-90031-sp (ATCC 90467) is available from ATCC, and the base plasmid used for experiments herein; pPHT1 (Cummings et al. ) was a kind gift of Professor Miriam E. Zolan, Department of Biology at Indiana University.

Techniques: Construct